By J. R. Chapman
John R. Chapman recreates the luck of his prior acclaimed e-book (Protein and Peptide research through Mass Spectrometry, 1996) with an immense new number of state of the art equipment within the research of proteins and peptides. Contributed by way of well-established investigators, every one bankruptcy offers historical past info ahead of guiding the researcher step by step in the course of the experimental technique, with certain directions to assist guarantee experimental luck. The purposes lined diversity generally and contain protein sequencing, higher-order constitution selection, epitope mapping, kinetics, quantitation, glycosylation research, and bacterial typing. Authoritative and eminently functional, Mass Spectrometry of Proteins and Peptides demonstrates the entire scope of this flexible analytical method. All protein chemists and biochemists, in addition to bioanalytical scientists, who are looking to make the most those robust equipment of their paintings, will locate this e-book crucial.
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Extra resources for Mass Spectrometry of Proteins and Peptides (Methods in Molecular Biology Vol 146)
Example text
3. 1. 1. General Description Lutefisk97 (8) is a computer program that produces a short list of sequence candidates from a tandem mass spectrum of a peptide (14). The program first Searching Sequence Databases 43 reads either unprocessed mass peak profile data, peak centroid data, or manually selected mass-to-charge (m/z) values, and derives a list of 30–70 ions that are considered significant. From these ions, a “sequence spectrum” (9), which is a graph in which all ions are mathematically converted to ions of a single type, is derived.
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Garcia, J. , et al. (1995) Protein reverse staining: high efficiency microanalysis of unmodified proteins detected on electrophoresis gels. Anal. Biochem. 224, 203–211. 21. Wilm, M. (1994) Electrospray and Taylor-cone theory, Dole’s beam of macromolecules at last? Int. J. Mass Spectrom. Ion Processes 136, 167–180. 22. Mann, M. , and Meyer, H. ), VCH, Weinheim, pp. 223–245. 23. , and Mann, M. (1998). High sensitivity analysis of gel separated proteins by a quadrupole-TOF tandem mass spectrometer, in Proceedings 46th ASMS conference on Mass Spectrometry and Allied Topics, Orlando, FL, p.