By Mary W. Trucksess, Albert E. Pohland
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Extra info for Mycotoxin Protocols (Methods in Molecular Biology Vol 157)
The amounts needed for most methods of analysis and for fortifying various matrixes for use as laboratory test or control samples, are in the nanogram or microgram ranges. The protocols given are for the aflatoxins B1, B2, G1, and G2. Mycotoxins standards other than the aflatoxins may be prepared in the same way using the information provided in Table 1. For some mycotoxins, such as the fumonisins and deoxynivalenol, UV spectroscopy cannot be used due to the lack of a suitable chromophore in the molecule.
2. 1. Fungal Culture Material The fungal culture material was prepared by the Fusarium Research Center, Pennsylvania State University (see Note 1). Briefly, a known fumonisin-producing Fusarium proliferatum was inoculated into 500 g yellow corn and 500 mL distilled water, after the mixture was autoclaved in polyethylene bags. The bags were incubated in the dark at 20–22°C for 4 wk. 2. Preparatory HPLC System 1. A programmable solvent delivery system capable of producing gradient mixtures of at least two solvents.
And Roberts, D. ), ACS Series 451. Amer. Chem. , Washington, D. , pp. 162–169. 35. Park, D. , Rua, S. , Paulson, J. , and Young, A. K. (1991) Sample collection and sample preparation techniques for aflatoxin determination in whole cottonseed. J. AOAC Int. 74, 73–75. 36. Whitaker, T. , and Nesheim, S. (1996) Variability associated with analytical methods used to measure aflatoxin in agricultural commodities. J. , 79, 476–485. Isolation of Mycotoxins 25 3 Preparatory Isolation of Mycotoxins from Solid Phase Fungal Cultures Robert M.