By Thomas E Schroeder; American Society for Cell Biology
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Extra resources for Methods in cell biology / Vol. 27, Echinoderm gametes and embryos / edited by Thomas E. Schroeder
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K. ), pp. 139-156. Crowell, New York. Hinegardner, R . T. (1975). In “The Sea Urchin Embryo, Biochemistry and Morphogenesis” (G. ), pp. 10-22. Springer-Verlag. Berlin. , and Kato, K. H. (1977). Acta Embryo/. Exp. 1, 21-32. Johnson, C. , and Epel, D. (1983). J. Exp. Zool. 226, 431-440. Johnson, C. , Clapper, D. , Winkler, M . , Lee, H. , and Epel, D. (1983). Dev. Biol. 98, 493-501. Kato, K. H.. and Sugiyama, M. (1983). Dev. Growth Direr. 25, 153-163. Kozhina, V. , Terekhova, T. V. I. (1978). Dev.
Pictus, S. purpuratus, and S . franciscanus sperm show essentially the same pattern of labeling of these four plasma membrane glycoproteins. The only differences found with A. puncrulata are that all four bands are of slightly higher apparent molecular weight and the band just above the 210,000 region is not as intensely labeled. B. Labeling with [ ~25I]DiiodofluoresceinIsothiocyanate (IFC) This probe was first synthesized by Gabel and Shapiro (1978). It was subsequently used for the isolation by immunoabsorption of labeled sperm components using antibodies to IFC (Gundersen and Shapiro, 1984a) and to study the fate of IFC-labeled sperm components in the embryo (Gundersen and Shapiro, 1984b).
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