By Thomas J. McIntosh (auth.), Thomas J. McIntosh (eds.)
In the previous a number of years major awareness has been given to the research of the houses and capabilities of lateral microdomains (rafts) in organic membranes. As defined within the evaluate bankruptcy of this publication, in addition to within the introductions to a few of the different chapters, there are various primary un- swered questions about the composition, constitution, dynamics, or even the very lifestyles of those membrane rafts. hence, various refined recommendations were used to review intact mobile membranes, in addition to version s- tems composed of particular lipids and proteins regarded as in rafts. The analyses of organic membranes have supplied precise info on intact microdomains, and the complementary experiences of version structures have given serious insights at the roles of particular lipid-lipid and lipid-protein interactions in raft formation and serve as. The chapters during this publication offer specified info on glossy equipment which are at the moment being hired to check lipid rafts.
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Extra info for Lipid Rafts
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18. , and London, E. (2006) Cholesterol precursors stabilize ordinary and ceramide-rich ordered lipid domains (lipid rafts) to different degrees: Implications for the Bloch hypothesis and sterol biosynthesis disorders. J. Biol. Chem. 281, 21,903–21,913. 19. Koynova, R. and Caffrey, M. (1995) Phases and phase transitions of the sphingolipids. Biochim. Biophys. Acta 1255, 213–236. 20. Kremer, J. , Esker, M. , and Wiersema, P. H. (1977) Vesicles of variable diameter prepared by a modified injection method.
Low Tm lipids typically contain unsaturated acyl chains, whereas high Tm lipids typically contain long saturated acyl chains (19). As in the case of experiments using 12SLPC, DPH is a suitable fluorescent probe. Because the concentration of tempo within the ordered domains is low, DPH residing in the ordered domains will be relatively shielded from tempo. Therefore, DPH fluorescence will be much stronger than in a homogeneous bilayer in which all lipids are mixed and much of the DPH is in contact with tempo.
Acad. Sci. USA 92, 10,104–10,108. 8. Macdonald, J. L. and Pike, L. J. (2005) A simplified method for the preparation of detergent-free lipid rafts. J. Lipid Res. 46, 1061–1067. 9. Sehgal, P. , Guo, G. , and Patel, K. (2002) Cytokine signaling: STATS in plasma membrane rafts. J. Biol. Chem. 277, 12,067–12,074. 10. Aronson, N. , Jr. and Touster, O. (1974) Isolation of rat liver plasma membrane fragments in isotonic sucrose. Methods Enzymol. 31, 90–102. 4 Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo Omar Bakht and Erwin London Summary Short-range fluorescence quenching has proven to be an effective method to detect the presence of coexisting ordered and disordered state lipid domains in model membranes.