By Arvid B. Maunsbach, Björn A. Afzelius
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''The booklet encompasses a wealth of data at the interpretation of electron micrographs - in a level of aspect that sometimes is understood simply at the paintings ground itself... This ebook has safeguarded very important facets of electron microscopy for destiny morphologists.''
--TRENDS IN telephone BIOLOGY
''This is a wonderful ebook that are meant to be within the palms of any new or skilled practitioner of electron microscopy. The writing is apparent and direct and the micrographs set the top ordinary of method, caliber, and value. it is going to set a brand new common of excellence in reinforcing the significance of the cautious and applicable use of electron microscopy in smooth cellphone biology. No university, collage, or existence technology library may be with no it.''
--DOODY'S PUBLISHING reports
''It has to be the main finished choice of artifacts to be published!...The energy of the ebook is within the transparent and succinct presentation of innovations; the big variety of micrographs illustrating the consequences; the varied examples of artifacts and different defects; the top of the range info given approximately specimen guidance for every instance; and the references on the finish of every chapter.''
--MICRON
''This beautifully illustrated assortment would definitely obtain very heavy use...''
ULTRAMICROSCOPY (1999)
''The actual price of this ebook is that it presents a identified and fine quality reference regular opposed to which one's personal paintings will be judged and significantly evaluated. it truly is of profit to all those that paintings within the box of organic transmission electron microscopy, in addition to the scientific box indicated within the title...''
PROCEEDINGS OF THE ROYAL MICROSCOPICAL SOCIETY (1999)
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Extra info for Biomedical Electron Microscopy. Illustrated Methods and Interpretations
Example text
1C A rat liver fixed for 2 hr in 2% glutaraldehyde in the same buffer solution as in Fig. 1 M phosphate buffer for 2 hr. Embedding and staining as in Fig. 1A. • 25,000. By comparing "single" and "double-fixed" tissues the impression is gained that double fixation retains more material in the mitochondrial matrix and in the cytoplasmic ground substance. This impression is supported by studies that show a greater extraction of lipids and proteins in tissues fixed in osmium tetroxide alone as compared to glutaraldehyde followed by osmium tetroxide.
Electron Microsc. Tech. 6, 255-301. Fawcett, D. W. " Saunders, Philadelphia. Harb, J. M. (1993). Interpretation of TEM micrographs for human diagnosis. Microsc. Soc. Am. Bull 23, 206-218. Hockley, D. , Wood, R. , Jacobs, J. , and Garrett, A. J. (1988). Electron microscopy of human immunodeficiency virus. J. Gen. Virol. 69, 2455-2469. Johannessen, J. V. (1977). Use of paraffin material for electron microscopy. Pathol. Annu. 12, 189-224. JCrgensen, P. L. (1974). Purification and characterization of (Na+-K+) ATPase.
One reason why a 10% formalin solution (which contains 4% formaldehyde) is a less than optimal fixative for electron microscopy is that it contains formic acid and other impurities and that it often does not contain solutes that protect cells from osmotic distortions. A better result can be expected from a buffered and osmotically balanced formaldehyde solution. In Figs. 14C the cell originates from an adrenal tumor, and the question was whether the tumor is a pheochromocytoma from the adrenal medulla or a cancer of the adrenal cortex.