By Christopher D. Richardson
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Extra info for Baculovirus Expression Protocols.djvu
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2 The Expansion of HSPCs . . . . . . . 1 Cytokines . . . . . . . . 2 Ex Vivo Expansion of HSPCs . . . . 3 Regulation of HSPCs Expansion . . . 5 Megakaryocytic Progenitor Cells Expansion . 6 Red Cells Expansion . . . . . . 7 T-Cell Expansion . . . . . . . 8 NK Cell Expansion . . . . . . 9 DC Expansion . . . . . . . 3 Expansion Bioreactor . . . . . . . . 4 The Application of Expanded HSPCs . . . . 1 Transplantation of HSPCs in Animal Model . 2 Transplantation of HSPCs in Human .
2001) No difference in graft-versus-host disease, relapse, and survival comparing peripheral stem cells to bone marrow using unrelated donors. Blood 98: 1739–1745. 79. Remberger M, Beelen DW et al. (2005) Increased risk of extensive chronic graft-versus-host disease after allogeneic peripheral blood stem cell transplantation using unrelated donors. Blood 105: 548–551. 80. Garderet L, Labopin M et al. (2003) Patients with acute lymphoblastic leukaemia allografted with a matched unrelated donor may have a lower survival with a peripheral blood stem cell graft compared to bone marrow.
Joshi et al. [91] additionally reported that IL-2-activated mononuclear cells from umbilical cord blood cells (UCBCs) had been shown increased cytotoxicity against K562 and Raji hematopoietic malignant cells compared with peripheral blood cells (PBCs). Human NK cells from HSC transplanted NOD/SCID mouse origin showed cytotoxicity against HLA class 1-deficient K562 targets either in vitro or in vivo [92]. Cavazzana-Calvo and colleagues [93] studied NK cell differentiation from cord blood CD34+ cells in the presence of SCF, IL-2, and IL-7, this cytokine combination efficiently induced CD34+ cord blood cells to proliferate and mature into NK cells.