By Xuhua Xia
Information research in Molecular Biology and Evolution introduces biologists to DAMBE, a proprietary, basic laptop software for molecular facts research. the original mixture of this booklet and software program will permit biologists not just to appreciate the motive at the back of numerous computational instruments in molecular biology and evolution, but additionally to achieve rapid entry to those instruments to be used of their laboratories. facts research in Molecular Biology and Evolution serves as an very good source for complex point undergraduates or graduates as good as for execs operating within the box.
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Additional info for Data Analysis in Molecular Biology and Evolution
Make sure that you formulate the keywords carefully to get what you want. An example of searching with keywords is illustrated in fig. 1. The search string tells DAMBE to retrieve the first 20 nucleotide sequences in GenBank that contain words “Geomys” and “cytochrome”. “Geomys” is the generic name for a group of small rodents called pocket gophers. It is simpler to search with the GenBank accession number or LOCUS name. Each sequence deposited in GenBank is associated with one LOCUS name and at least one accession number.
Enter the accession numbers listed in the previous paragraph into the text box, separated by a comma, as shown in fig. 1. Click the Retrieve button and the 21 sequences will be retrieved in GenBank format. When the retrieval is complete, which may take several minutes, you will be prompted to save the retrieved sequences. GB file type as default. In the dialog box that comes next, click the CDS option and then click the Proceed button. , the nucleotide sequence that specifies the amino acid sequence.
Only part of the dialog box is shown. Let me explain each item briefly. Each of the 21 sequences has a LOCUS name, which is shown in the first listbox (fig. 2). The second list box shows the length of the sequence for 52 Chapter 7 each LOCUS. The third column is descriptive, taken from the DEFINITION clause of the GenBank sequence. The right side of fig. , CDS for coding sequences. There are also two listboxes (not shown) on the right of the dialog. These listboxes will show additional information when we click a LOCUS name in the first column.