By Milton H. Saier Jr., Gary R. Jacobson (auth.)
Man's brain stretched to a brand new inspiration by no means is going again to its unique dimensions Oliver Wendell Holmes Our present realizing of intercourse and organic differentiation effects from the applying of 3 central experimental ways to those matters: these of the physiologist, the biochemist, and the geneticist. those 3 methods are illustrated by means of the fabrics awarded within the chapters of this quantity. Chapters 1-5 emphasize conceptualization of developmental strategies, describing structures largely from the viewpoint of the physiologist. constructions and services are outlined with merely occasional connection with particular molecular info. Chapters 6- 10 current the perspectives of the biochemist, trying to describe features influ encing or regulating mobile habit on the molecular point. And Chapters eleven- 14 illustrate the ways of the modem-day geneticist in his makes an attempt to realize a close figuring out of techniques controlling gene expression. whereas it truly is attainable to delineate those 3 significant sections, every one emphasizing a unique experimental technique, it has to be discovered that the yield of information raises exponentially with the variety of experimental methods to be had to the investigator. details because of the appliance of every of those techniques needs to converge to provide an analogous solutions for someone organic phenomenon in somebody experimental procedure. additional, if we will research of information concerning a selected method through making use of various experimental techniques, our postulates about the underlying molecular mechanisms usually are extra exact. yet organic structures should not unrelated.
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Additional resources for The Molecular Basis of Sex and Differentiation: A Comparative Study of Evolution, Mechanism and Control in Microorganisms
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In contrast, fluorescent lysozyme (FLy), a basic protein, is observed to remain within the nurse cells after injection. This is explained by the fact that the oocyte cytoplasm is about 10 mV more positive than that of the nurse cells. Reprinted by permission from Nature , Vol. 286, pp. 84-86. Copyright © 1980 Macmillan lournals Limited. Although we have limited our discussion of the role of ion currents in development to these two well-studied examples, it is probable that such currents as well as more transient electric fields associated with changes in membrane potentials will have general importance in influencing cellular differentiation and growth in the biological world.
Fresh spores do not germinate easily, but anyone of a number of treatments "shock" or activate them so that germination is facilitated. Aging for several weeks, sublethal heating, or exposure to low pH, sulfhydryl-containing compounds, or organic solvents such as dimethylformamide serve to activate the spore. Activation is required for fresh bacterial endospores, but not for germination of bacterial or eukaryotic conidiospores or spores derived from fruiting bodies. Numerous agents have been found to initiate germination of activated endospores, and these are to some extent species-specific.
In contrast, if cells were not aligned by light, no current was driven through the tube (B). Current patterns around the cells that were inferred from these experiments are shown by the curved arrows. (Adapted from L. Jaffe, Proc. Natl. Acad. Sci. 8. Use of a vibrating platinum electrode to measure current patterns around a developing Pelvetia egg. (A) Micrograph of the vibrating electrode (arrow shows the axis of vibration) near a Pelvetia egg 3 hr after fertilization. (B) St~ady current pattern inferred by making measurements with the probe at various position~ around the egg cell.