By E. Johns (Eds.)
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H . , W o o d h e a d , L . a n d J o h n s , E . W . ( 1 9 7 7 ) . FEBS Lett. 7 3 , 8 5 - 8 8 . Goodwin, G . H . , W a l k e r , J . M . a n d J o h n s , E . W . ( 1 9 7 8 ) . In " T h e Cell N u c l e u s " ( H . B u s c h , e d . ) , Vol. V I , p p . 1 8 2 - 2 1 9 . A c a d e m i c P r e s s , N e w Y o r k a n d L o n d o n . G o r d o n , C . N . ( 1 9 7 7 ) . J. Cell Science 2 4 , 8 1 - 9 3 . G o r d o n , J . , Rosenfeld, Β . I . , K a u f m a n , R . a n d Williams, D . L . ( 1 9 8 0 ) .
J. 177, 917-923. P e t e r s e n , L . G. H . a n d Sheridan, W . F . ( 1 9 7 8 ) . Carlsberg Res. Commun. 4 3 , 4 1 5 422. R a b b a n i , A . ( 1 9 7 9 ) . P h . D . thesis, L o n d o n U n i v e r s i t y . R a b b a n i , Α . , Goodwin, G . H . and J o h n s , E . W . ( 1 9 7 8 a ) . Biochem. J. 1 7 3 , 4 9 7 - 5 0 5 . R a b b a n i , Α . , Goodwin, G . H . a n d J o h n s , E . W . ( 1 9 7 8 b ) . Biochem. Biophys. Res. Commun. 8 1 , 3 5 1 - 3 5 8 . R a b b a n i , Α . , Goodwin, G . H . , W a l k e r , J .
35 Μ NaCl extracts of pig-thymus chromatin. 4. T h e use o f T C A precipitation a n d C M - c e l l u l o s e c h r o m a t o g r a p h y a t p H 5 - 5 . 8 column are still contaminated with other proteins and a further purification step is required. Sometimes a simple T C A fractional precipitation is sufficient. Thus H M G 1 and 2 are quantitatively precipitated from aqueous sol utions with 1 0 % T C A , H M G 14 is partially precipitated at 1 2 % T C A , and H M G 17 is completely soluble in 1 2 % T C A .