By Sankar Adhya (Editor), Susan Garges (Editor)
RNA polymerase is molecule vital to gene transcription. in addition to linked elements, RNA polymerase is a part of the method during which RNA is transcribed to provide a protein. * versions and strategies for learning polymerase translocation* Assay for activities of RNA polymerase alongside DNA* Engineering of elongation complexes of bacterial and yeast RNA polymerases
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A. Campbell, L. Minakhin, C. Richter, K. Severinov, S. A. Darst, Cell 98, 811–824 (1999). 6 P. Cramer, D. A. Bushnell, R. D. Kornberg, Science 292, 1863–1876 (2001). 2 METHODS IN ENZYMOLOGY, VOL. 371 Copyright 2003, Elsevier Inc. All rights reserved. 7 In addition to structures which keep the polymerase from releasing the DNA, we also considered that interactions with DNA and RNA might limit the range of sliding. For example, base pairing between DNA and RNA and interactions between RNA and polymerase would keep the polymerase from sliding away from the 30 -end of the RNA in the downstream direction, while extensive upstream sliding could be restricted if the template binding cleft immediately downstream of the active site (which normally binds single-stranded template) is unable to accommodate an RNA:DNA hybrid.
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