By Abelson J. (ed.), Simon M. (ed.), Wu R. (ed.)
The looks of one other quantity in that first-class sequence, tools in Enzymology, is usually a reason for appreciation should you desire to effectively perform a specific process or organize an enzyme or metabolic intermediate with out the tiresome prospect of looking through strange literature and maybe picking an unproven process which isn't simply reproduced.
Read or Download Recombinant DNA Part G PDF
Similar biology books
Biology and Regulation of Blood-Tissue Barriers
This e-book used to be written through many extraordinary investigators who've spent many years to check various features of blood‑tissue barrier functionality. they've got summarized a number of the newest and engaging improvement of their fields of study together with the blood‑brain barrier, the blood‑retinal barrier, the intestine barrier, the blood‑biliary barrier, the blood‑follicle barrier, the blood‑epididymis barrier, the blood‑testis barrier, the tight junction barrier more often than not in addition to boundaries within the woman reproductive tract.
- Mechanisms of Leukocyte Activation
- Advances in Enzymology and Related Areas of Molecular Biology, Volume 9
- Enzyme Kinetics. Principles and Methods
- International Review of Cytology, Vol. 88
- Advances in Microbial Physiology, Vol. 17
- Enzyme-Catalyzed Synthesis of Polymers APS 194-29
Additional resources for Recombinant DNA Part G
Sample text
Resuspend the bacterial pellet thoroughly by vortexing in 200/xl of TE buffer. Place in a boiling water bath for 1 min. 2~ 2. 5 M NaCI]. Vortex for 15 sec. Add 400/zl of a 1 : 1 phenol-chloroform mixture. 23 Vortex vigorously for 15 sec. Spin at full speed in a microfuge for 15 min at room temperature. 3. 5-ml microfuge tube. Add an equal volume of chloroform, vortex for 10 sec, and spin at room temperature for 30 sec to separate the two layers. 5-ml microfuge tube. Precipitate the plasmid DNA by mixing with an equal volume of ice-cold 2-propanol.
Spin at full speed for 5 min in a microfuge at room temperature to separate the aqueous and organic layers. 5-ml microfuge tube, and extract with 400/~1 of chloroform. Spin at full speed for a few seconds in a microfuge at room temperature. 5-ml microfuge tube. Precipitate the nucleic acids by adding 800 /~1 ethanol and mixing well. Store for 30 min at - 7 0 °. Spin down for 15 min at full speed in a microfuge at 4°. 9. Dissolve the DNA pellet in 20/zl of TE [TE: 10 mM Tris-HCl (pH 17 Please note that the uncut plasmids tested here will exhibit multiple bands corresponding to different topological forms.
MI3 DNA was isolated as in protocol 3. Sequencing reactions were performed using the KiloBase Sequencing System (Bethesda Research Laboratories) according to the manufacturer's instructions. Reaction products were resolved on a 6% polyacrylamide-8 M urea gel at 60 W in Tris-borate EDTA buffer. The first four lanes (left-hand side) are reactions using DNA isolated by standard phenol extraction procedures (Sambrook e t a l . ) . 4 The other four lanes (right-hand side) are reactions using DNA captured and released from the capture reagent.