By W Hennig
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The Wt1 knockout mice have fewer mesonephric tubules. Other than this, I don’t think much work has been done on the mesonephros. Short: Does the genital ridge form normally in these mice? 5. This is very similar to what we see in the ÀKTS knockout. Koopman: What is the e¡ect of ectopic expression of Sox9 in the kidney? Schedl: There is no e¡ect. The mice seem to be completely normal. In the kidney Sox9 is expressed at the ureteric tip, whereas Wt1 is expressed in the metanephric mesenchyme. I think Sox9 has to work in the epithelial component, at least in the kidney.
Its disruption results in various degrees of sexual anomalies. Sexual development may be viewed as being composed of two processes: sex determination and sexual di¡erentiation. This distinction, although somewhat arti¢cial, has nevertheless proved important to the understanding of the medical classi¢cation and management of abnormalities of human sexual development. Sex determination is the developmental decision that directs the orientation of the undi¡erentiated embryo into a sexually dimorphic individual.
Harley: We have data showing that a couple of sex-reversing campomelic dysplasia (CD) mutations do a¡ect stability, and this in turn a¡ects its ability to be recognized by its nuclear receptor importin b and be translocated into the nucleus. Regardless of DNA binding activity, if it is not getting into the nucleus it can’t do its job. Lovell-Badge: Is anyone aware of mutations outside the HMG box that are really having an e¡ect on its potential DNA binding activity? Harley: I have measured two sex-reversing mutations in SRY, one in the N-terminal region and the other in the C-terminal region, and neither have e¡ects on DNA binding or bending.