By Gerhard W. Hacker, Raymond R. Tubbs
Molecular Morphology of Human Tissue with gentle Microscopy provides a variety of molecular morphological options for use in scientific diagnostics, biomedical examine and biotechnology. Written by way of specialists within the box, the ebook contains lately pronounced "futuristic" methodologies, corresponding to the three-dimensional demonstration of complete colour microscopic photographs got in basic vivid box microscopy, which doesn't rely on the necessity for expensive laser-equipped equipment (such as confocal microscopes) and for the 1st time offers 3D copy of the full spectrum of transmitted and fluorescent gentle illumination.
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Extra info for Molecular Morphology in Human Tissues: Techniques and Applications (Advances in Pathology, Microscopy, & Molecular Morphology)
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The sensitivity of the S-ABC method can be further increased by the catalyzed reporter deposition (CARD) method, either directly using a fluorophore-conjugated 10 tyramide, or indirectly using a biotinlabeled tyramide visualized by fluorophore-conjugated streptavidin [68, 69]. The CARD method has also been termed “tyramide signal amplification” (TSA; Perkin-Elmer Life Sciences Products, Boston, MA) or the “catalyzed signal amplification” (CSA; DakoCytomation) method. 6, Protocols IV and V). In co-localization studies, immunofluorescence techniques, used in double or triple staining performed on the same section, are superior to all other techniques because the substances can be determined more exactly and without any interference in individual cells.
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125, 279,1989. 70. M. et al,. Co-localization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract, Histochem. , 111, 49, 1999. 71. M. , Expression of the five somatostatin receptor subtypes in endocrine cells of the pancreas, Appl. Immunohistochem. Molec. , 8, 126, 2000. 1702_C001 11/17/04 10:51 AM Page 25 Immunostaining Techniques for Co-Localization of Multiple Peptide Antigens 72.