By Gustave Flaubert
For bold to look into the guts of an adulteress and enumerate its contents with profound dispassion, the writer of Madame Bovary used to be attempted for "offenses opposed to morality and religion." What shocks us this present day approximately Flaubert's devastatingly learned story of a tender lady destroyed through the reckless pursuit of her romantic goals is its natural artistry: the poise of its narrative constitution, the opulence of its prose (marvelously captured within the English translation of Francis Steegmuller), and its production of a global whose minor figures are as very important as its doomed heroine. In interpreting Madame Bovary, one studies a piece that is still really progressive nearly a century and a part after its creation.
Translated through Francis Steegmuller.
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Additional resources for Madame Bovary
Sample text
Anyone faced with nonlinear Scatchard plots after considering the points discussed above (Sects. f. 1-5) should use these sources for a thorough analysis of interacting systems. 3. Receptor States and 3H-Steroid Exchange Most biologic systems contain both occupied and unoccupied receptors. The assessment of both forms is important if an· accurate picture of the relationship between steroid binding and physiology is to emerge. , 1972a). , 1973a). Subsequently the 3H-steroid exchange technique has been widely employed (for references, see Sects.
1971). , 1975a). Although these procedures are rapid and generally effective for the separation of free from bound S, they are not without fault. One should employ them cautiously, always bearing in mind the dangers inherent in determining equilibrium values under nonequilibrium conditions. As discussed earlier, both occupied and unoccupied sites can be measured by 3Hestradiol exchange. , 1972a) was modified for the measurement of cytosol-estrogen receptors by Katzenellenbogen et al. (1973).
Saturation analysis is performed as described above for the 3H-estradiol exchange assay. The nuclear fractions are incubated with shaking at 4°C for 18-24 h. 5 ml TO buffer is added, and the samples are centrifuged at 800 g for 10 min. 0 ml ethanol. The radioactivity in the ethanol extract is determined as explained for the 3fI-estradiol exchange procedure. 3) Nuclear Binding and Retention of Receptor-Hormone Complexes The interactions between RnS complexes and nuclear binding sites are poorly understood.