By Alan J. Lacey
This absolutely up-to-date version surveys either outdated and new options, together with chromosome microscopy, immunohistochemistry, fluorescence microscopy, snapshot development, video microscopy, calcium and pH imaging, microinjection strategies, nanovid microscopy, and histomorphometry.
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Additional resources for Light Microscopy in Biology: A Practical Approach
Example text
When returning to a lower NA objective remember to again check steps 5-9. To complete the understanding of the conjugate planes in the microscope place a piece of ground glass or lens tissue at right angles to the light path just above the eyepiece. Move the tissue or glass along the light path towards or away from the surface of the eyepiece. You 8 1: Basic optical microscopy will discover a place where the apparent disc of light is at a minimum dimension. This disc of light is the exit pupil of the microscope, otherwise known as the Ramsden disc.
Determination of permitted vibration direction Equipment Pieces of Polaroid Preferably a polarized light microscope with a rotating stage and slots (but sufficient for this protocol would be two pieces of Polaroid) 23 (About) 20 x 10 cm of glass 3 mm or more thick High intensity light source Section of coniferous wood unstained or very weakly stained A. J. Lacey Protocol 8. Continued A. To determine the permitted vibration direction of a piece of unknown Polaroid from first principles Principle: when an unpolarized beam of light is reflected off a transparent surface the reflected beam is plane polarized and its vibration direction is parallel to that of the surface of the reflecting material and at right angles to the ray direction (see Figure 8).
39 Table 2. 13/4 and 4a) (Fig. d.