Laser Scanning, Theory and Applications by Chau-Chang Wang (Ed.)

By Chau-Chang Wang (Ed.)

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In a clinical study comprised of 20 healthy mothers (25-43 years old) and their biological children (3-24 months old), the skin microstructure of the lower thigh area of infants and adults were examined and compared. 5 μm in step size. The lower thigh area was examined for ease of measuring infants, as it is difficult to keep them voluntarily still. The mothers held the infants in their laps throughout imaging. Although there was still some movement in the image stacks, every effort was taken to prevent movement of subjects during imaging.

Fig. 11. Representative cross-polarized images of the micro-wounds at various posttreatment time points along with confocal images 50 and 120 μm below the top of the SC. Intensity profiles are also shown. 7. Summary and conclusions Confocal laser scanning microscopy is a novel technique that enables us to non-invasively investigate skin at the cellular level and dynamic changes that may occur over a period of time. It was shown that the incident light wavelength in CSLM systems affects signal intensity and structural details at the cellular level.

P. W. Stachowiak, Characterization of Surface Topography of Wear Particles by SEM Stereoscopy, Wear, Vol. 206, 1997, pp. 39-52. Sheppard CJR and Matthews HJ (1988), The extended-focus, auto-focus and surfaceprofiling techniques of confocal microscopy, Journal Of Modern Optics, Vol. 35, No. 1, pp. 145-154. Sheppard CJR and Shotton DM (1997), Confocal Laser Scanning Microscopy, BIOS Scientific Publishers, Oxford, pp 75. Scott PJ (2009), Feature parameter, Wear, Vol. 266, pp. 548-551. Webb RH (1996), Confocal optical microscopy, Rep.

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