By Gian Antonio Danieli (auth.)
Genetics and Genomics for the Cardiologist is a concise, yet finished quantity for the medical heart specialist or clinical pupil drawn to studying how molecular genetics is now being utilized to prevention and remedy of center illnesses, from DNA exams to pharmacogenomics and gene-based therapeutics. the quantity, written in a simple language, comprises designated figures. A wealthy thesaurus, 3 appendices, many references and several other URLs offer extra resources of information.
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Extra resources for Genetics and Genomics for the Cardiologist
Sample text
The position of each DNA sample is automatically recorded. After having been labeled with a fluorescent marker, a mixture of cDNA molecules obtained by retrotranscription from the selected tissue is hybridized to the array. Minispots of fluorescence are detected in correspondence of single points of the array, by laser scanning. Results are automatically transformed into digital outputs and analyzed by statistical software. In reality each array is hybridized both with test cDNA and with control cDNA, marked with different fluorescent markers.
E, unless it would be subjected to strong perturbations affecting transcription levels of genes coding for functional nodes of such network. 4 The transcriptome of the human heart The first compendium of genes expressed in the cardiovascular system was produced by Hwang in 1997 (Tab. 1). 1: An estimate of relative distribution of functions among genes active in the human cardiovascular systems (according to Hwang, 1997) Functional category No. 00 More recently, a small subset of such catalogue appeared in a publication by the same Author, where the correspondence of in silico predictions with 45 experimental data on gene expression in heart was demonstrated (Hwang, 2000).
Tissue samples of small or very small size. At present, it is possible to monitor with high confidence only variations in the expression of a limited number of genes which appear highly expressed in the heart (Barrans, 2001). The distinction between genes expressed in myocardial cells and those active in coronary vessels or in other cells of cardiac tissue is at the moment impossible; however data on differential gene expression in different components of the heart may be obtained from alternative sources (in-situ RT-PCR, immunohistochemical staining, etc).