Genetics and Biotechnology of Bacilli by A. T. Ganesan

By A. T. Ganesan

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C] Sub-fragments of shuttle vector pMS102 the BamHI fragments recloned in pBR322 that were used as the probes for plaque hybridization. [Dj Fragments cloned in Charon 28. B . S t r u c t u r e a n d Function o f r R N A O p e r o n s The t w o rRNA gene sets in t h e o r i g i n r e g i o n , e s p e c i a l l y the o n e n e a r e s t t h e o r i g i n , m a y play a n i m p o r t a n t role in i n i t i a t i o n o f r e p l i c a t i o n . It w a s , t h e r e f o r e , o f i n t e r e s t to d e t e r m i n e t h e s t r u c t u r e a n d t r a n s c r i p t i o n o f t h e s e gene sets.

Y o s h i k a w a , a n d t h e n m a p p e d its p o s i t i o n b e t w e e n gauA a n d purA. The c o l l e c t i o n of c l o n e d D N A u s e d in o u r studies is s h o w n in Figure 3. , 1982) in light of d i r e c t s e q u e n c e a n a l y s i s . N o w t h a t w e k n o w t h e D N A sequence of t h e p l a s m i d p l 4 B 8 spacer r e g i o n f r o m Peter Zuber's d i s s e r t a t i o n (University o f V i r g i n i a , 1982) w e c a n be sure t h a t t h i s c l o n e is t h e o n e 27 RIBOSOMAL RNA GENES Table 2.

Natl. A c a d . Sei. USA 78, 4 9 3 6 . H a l d e n w a n g , W . G . , O ' C o n n o r , M . B . a n d S o n e n s h e i n , A . L . ( 1 9 8 0 ) . J . Bacteriol. 142, 9 0 . J. A. ( 1 9 8 0 ) . M i c r o b i o l . Rev. 44, 5 7 . K o b a y a s h i , H . a n d O s a w a , S. ( 1 9 8 2 ) . FEBS Letts. 141, 1 6 1 . E. E. ( 1 9 8 2 ) . Nucl. A c i d s R e s . , 1 6 0 7 . , B r a d y , C. a n d Rosenberg, M . (1981). _In^ Gene A m p l i f i c a t i o n a n d A n a l y s i s , V o l . G. C h i r i k j i a n a n d S.

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