By Jichun Tian, Zhiying DENG, Kunpu Zhang, Haixia Yu, Xiaoling Jiang, Chun Li
The ebook in general describes the QTL mappings and efficacy analyses which are linked to wheat productiveness, caliber, body structure and diverse rigidity resistances and offers summaries of effects from reports carried out either at domestic and out of the country. It offers related information and analyses, supporting readers to reach at a extra accomplished knowing of the most recent improvement during this box. The e-book presents a wealth of novel info, huge diversity of functions and in-depth findings on crop genetics and molecular breeding, making it useful not just for plant breeders but additionally for tutorial schools, senior researchers and complicated graduate scholars who're all for plant breeding and genetics. Dr. Jichun Tian is a professor on the division of Agronomy, Shandong Agricultural college, Tai’an, China.
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Extra info for Genetic Analyses of Wheat and Molecular Marker-Assisted Breeding, Volume 1: Genetics Map and QTL Mapping
Example text
An ideal SSSL should maintain all recipient genetic background with the exception of the targeted QTL DNA segment that comes from the donor’s chromosome, strongly suggesting that SSSL can be used for fine mapping of single QTL. However, during the backcross process, the QTL gained via the preliminary mapping should be used for assistance selection, resulting in issues such as labor intensive and tedious. Chromosome Segment Substitution Line Different from SSSL, CSSLs are a series of NILs in which the substituted segments of the wide population contain the entire information of the donor, while each CSSL carries one or more donor chromosome segments in the genetic background of the recipient.
The amplified product is about 200–500 bp DNA sequence occurring only once in the genome and can be used for locating specific gene sites (Olson et al. 1989). Compared with RFLP, STS marker is most advantageous as it does not need to maintain the probe clones, and obtaining the sequence information 30 2 Genetic Analysis Methods of Quantitative Traits in Wheat from the database is simple and easy. Moreover, the STS marker is codominant and marker information can be shared within genetic profiles of various crops.
It combines the advantages of both RFLP and RAPD markers. The digested genomic DNA fragments with the two types of restriction nucleases produce sticky ends which are ligated with the synthetic double-stranded DNA. A subset of the restriction fragments is then selected to be amplified. The amplified fragments are separated and visualized on denaturing polyacrylamide gels, either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments. The marker has the advantages of simple and fast, high polymorphism and good stability and therefore can be used to study the genetic diversity of germplasm resources, genetic map construction, and gene mapping.