By Amanda S. Coutts, Louise Weston
This quantity brings jointly a special selection of protocols that hide commonplace, novel, and really good concepts. Cell Cycle Oscillators: equipment and Protocols courses readers via fresh growth within the box from either holistic and reductionist views, supplying the most recent advancements in molecular biology thoughts, biochemistry, and computational research used for learning oscillatory networks. Written within the hugely winning Methods in Molecular Biology series structure, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and pointers on troubleshooting and heading off recognized pitfalls.
Authoritative and state-of-the-art, Cell Cycle Oscillators: tools and Protocols will function a useful connection with achieve additional perception into the complicated and incompletely understood strategies which are all for the phone cycle and its law through oscillatory networks.
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Sample text
6 Reconstituting Cell Cycle Events in Cell-Free Extracts of Frog Xenopus laevis Eggs The reconstitution of a biological process in a cell-free system is an ultimate goal of biochemical research to study the mechanism of a biological process at a molecular level. The frog egg has several unequaled advantages for such an approach. It is essentially pure cytoplasm, containing stockpiles of cellular components. It arrests as a single state of the cell cycle, either in G2 phase as an immature oocyte or in M-phase as a mature egg, and it can be made to go from one state to the other state of the cell cycle by adding purified molecules.
For example, the initiation of mitosis must be coupled to the completion of DNA replication, in order to exert the checkpoint control on the precise phase transitions. All eukaryotic cells synthesize their DNA during S phase, replicate DNA only once per cell cycle, and distribute the resulting identical copies of genetic information to daughter cells at mitosis. When mammalian cells such as baby hamster cells in S phase are treated with an inhibitor of DNA synthesis, for example, aphidocolin or hydroxyurea, the cells halt in S phase and will not progress into mitosis until the inhibitor is removed and DNA replication has been completed.
The sperm nuclei undergo decondensation in interphase and condensation at the onset of the 44 Zhaohua Tang Fig. 14 Reproducing mitotic cell cycle in a cell-free system of frog egg extracts.