By P. John, E. A. Reynolds, A. G. Prescott, A.-D. Bauchot (auth.), A. K. Kanellis, C. Chang, H. Klee, A. B. Bleecker, J. C. Pech, D. Grierson (eds.)
The inflorescence of the monoecious maize plant is exclusive one of the Gramineae within the sharp separation of the female and male constructions. The male tassel on the terminus of the plant normally sheds pollen earlier than the visible visual appeal of the receptive silks of th the feminine ear at a lateral bud, mostly on the 10 leaf [I]. past reviews tested the ontogeny of the growing to be tissues starting with the embryo within the kernel via to the most obvious protuberances of the becoming element because the kernel germinates. The differentiated constructing soon-to-become tassel and the lateral bulges that turn into the ears at the lateral buds develop into obvious very early within the germinating kernel [2, three, 46]. a undeniable variety of cells are destined for tassel and ear improvement . because the plant develops, there's a section transition [\3, sixteen] from the vegetative lateral buds to the reproductive lateral buds. this variation in part has been ascribed to genotypic regulate as evidenced within the variations between diversified genotypes within the initiation of the reproductive [I]. The genetic keep watch over of tassel and ear initiation has been gleaned from anatomical observations. Lejeune and Bernier [I2] stumbled on that maize vegetation terminate the initiation of extra axillary meristems on the time of tassel initiation. this is able to point out that the top-most ear shoot is initiated at the similar day because the initiation of tassel improvement and this occasion indications the tip of the undifferentiated turning out to be point.
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Extra info for Biology and Biotechnology of the Plant Hormone Ethylene II
5 kb untranscribed region of Vr-ACS6 contains all the elements sufficient to confer the hormonal interaction affecting Vr-ACS6 gene expression. 3. 1. ISOLA nON OF Vr-ACS6ISOGENE Using Vr-ACS6 eDNA, we screened a genomic library of mung bean, and nine positive clones were isolated after 3 rounds of screening. 6kb) was selected and used for further analysis. The nucleotide sequence of the gene contained a sequence identical to that of the cDNA, that were separated into three exons. The transcription initiation site determined by primer extension was the cytosine base 280 base-upstream of the translation initiation codon, ATG.
4. 1. Vr-ACS6 IS THE AUXIN-INDUCIBLE ISOGENE, BUT Vr-ACSI IS NOT Northern blot analysis was carried out with hypocotyl sections of etiolated mung bean (Vigna radiata) seedlings. The results indicated that mRNAs for Vr-ACSI and 6 were not detected in control hypocotyls, but incubation of hypocotyl sections with IAA induced great accumulation of Vr-ACS6 mRNA, but not of Vr-ACSI mRNA. 2,4dichlorophenoxyacetic acid, I-naphthaleneacetic acid and indole-3-butyric acid similarly induced expression of Vr-ACS6, but none of BA, ABA, methyljasmonate, salicylic acid, and sucrose induced expression of Vr-ACS6.
Conversely, antimorphic mutations are sensitive to gene dosage, and so if the ratio of wild-type to mutant genes is increased, a lessening of the phenotype is observed. Additionally, if eta3 is hypermorphic, a +/+/eta3 plant should have a wildtype phenotype. To distinguish these possibilities, we constructed a series of lines that contained different doses of the mutant allele of eta3 relative to the wild-type allele. To this end, we crossed a homozygous eta3 line to a wild-type diploid (Columbia) and tetraploid line (CS3151) and then measured ethylene production from the F I progeny, as well as from the parental lines (Table 3).