By Dr Desmond S. T. Nicholl
During this 3rd variation of his well known undergraduate-level textbook, Des Nicholl recognises sound take hold of of uncomplicated rules is essential in any advent to genetic engineering. consequently, in addition to being completely up to date, the e-book additionally keeps its specialise in the basic rules utilized in gene manipulation. The textual content is split into 3 sections: half I presents an creation to the appropriate simple molecular biology; half II, the tools used to govern genes; and half III, purposes of the expertise. there's a new bankruptcy dedicated to the rising value of bioinformatics as a different self-discipline. different extra beneficial properties comprise textual content packing containers, which spotlight very important features of themes mentioned, and bankruptcy summaries, which come with goals and studying results. those, in addition to key observe listings, suggestion maps and a thesaurus, will let scholars to tailor their learn to fit their very own studying types and eventually achieve a company clutch of a topic that scholars normally locate tricky.
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Additional info for An introduction to genetic engineering
Genes have been studied since the turn of the century, when genetics became established. Before the advent of molecular biology and Three important types of RNA are ribosomal RNA (rRNA), messenger RNA (mRNA), and transfer RNA (tRNA). 16:16 19 P1: SBT 9780521850063c02 20 CUUS128/Nicholl 978 0 521 85006 3 March 11, 2008 THE BASIS OF GENETIC ENGINEERING The gene is the basic unit of genetic information. Genes are located on chromosomes at a particular genetic locus. Different forms of the same gene are known as alleles.
One way of tracing the material is to label the nucleic acid with a marker of some sort, so that the material can be identiﬁed at each stage of the procedure. So what can be used as the label? 1 Types of label – radioactive or not? Radioactive tracers have been used extensively in biochemistry and molecular biology for a long time, and procedures are now well established. The most common isotopes used are tritium (3 H), carbon-14 (14 C), sulphur-35 (35 S), and phosphorus-32 (32 P). Tritium and 14 C are low-energy emitters, with 35 S being a ‘medium’-energy emitter and 32 P being a high-energy emitter.
Although DNA structure is a complex topic, particularly when the higher-order arrangements of DNA are considered, a simple representation will sufﬁce here, as shown in Fig. 5. 4 nm Fig. 5 The double helix. This is DNA in the commonly found B-form. 4 nm, with 10 base pairs per turn. The sugar–phosphate ‘backbones’ are antiparallel (arrowed) with respect to their 5 →3 orientations. One of the sugar–phosphate chains has been shaded for clarity. The purine–pyrimidine base pairs are formed across the axis of the helix.