By Cooper C., Packer N.
Proteome structures Ltd, North Ryde, Australia. Describes quite a few amino acid research ideas and the way every one method can be utilized to reply to particular biologic questions.
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Additional resources for Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159)
Williams, K. , and Gooley, A. A. (1994) Characterization of O-linked glycosylation motifs in the glycopeptide domain of bovine κ-casein. Glycobiology 4, 837–844. 15. Garnick, R. , Solli, N. , and Papa, P. A. (1988) The role of quality control in biotechnology: an analytical perspective. Anal. Chem. 60, 2546–2557. 16. Lundell, N. and Schreitmüller, T. (1999) Sample preparation for peptide mapping — a pharmaceutical quality-control perspective. Anal. Biochem. 266, 31–47. 17. Jones, M. , Merewether, L.
Morris, H. , Eteinne, A. , Rogers, M. , et al. (1991) A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator. Glycobiology 1, 173–185. Purification of Proteins for AAA 31 3 Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis Li Zhang and Nancy Denslow 1. Introduction Amino acid analysis of proteins is one of the best and most accurate methodologies to quantify proteins.
Cysteine Alkylation Monitoring 1. 1. 2. After removal of the acid, reconstitute the samples with Na-S buffer, and analyze using the standard program (Table 1) (see Note 25). Representative chromato- 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 20 Macchi et al. Fig. 2. Summary sheet using the AAA macro. Average compositions are given in the right-hand column, and the average mg/mL value is provided in the lower right hand box. CyA refers to cysteic acid, which is present when samples are oxidized intentionally.